MIA2 anticorps (Biotin)
Aperçu rapide pour MIA2 anticorps (Biotin) (ABIN181709)
Antigène
Voir toutes MIA2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Affinity chromatography
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Immunogène
- Highly pure (> 98 %) recombinant human MIA-2
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Indications d'application
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ELISA: Direct: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanMIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.
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Buffer
- PBS, pH 7.2
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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Date de péremption
- 6 months
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- MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))
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Autre désignation
- MIA2
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Sujet
- MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.Synonyms: Melanoma inhibitory activity protein 2
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ID gène
- 9606
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UniProt
- Q96PC5
Antigène
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